VyCAP's puncher technology for single cell identification, isolation, and analysis
نویسندگان
چکیده
منابع مشابه
Single Cell Isolation and Analysis
Individual cell heterogeneity within a population can be critical to its peculiar function and fate. Subpopulations studies with mixed mutants and wild types may not be as informative regarding which cell responds to which drugs or clinical treatments. Cell to cell differences in RNA transcripts and protein expression can be key to answering questions in cancer, neurobiology, stem cell biology,...
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Single-cell gene expression analysis holds great promise for studying diverse biological systems, but methodology to process these precious samples in a reproducible, quantitative, and parallel fashion remains challenging. Here, we utilize microfluidics to isolate picogram and subpicogram mRNA templates, as well as to synthesize cDNA from these templates. We demonstrate single-cell mRNA isolati...
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In this study, twenty-five whey samples collected from dairy industries in the city of Isfahan. The sampleswere cultured on malt extract broth (MEB) and yeast extract glucose chloramphenicol agar (YGCA) media.Eleven yeast strains (designated M1 to M11) were isolated from the culture. The strains were identified bytheir morphological and physiological properties. Betagalactosid...
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The handling of single cells is of great importance in applications such as cell line development or single-cell analysis, e.g., for cancer research or for emerging diagnostic methods. This review provides an overview of technologies that are currently used or in development to isolate single cells for subsequent single-cell analysis. Data from a dedicated online market survey conducted to iden...
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Plant growth promoting rhizobacteria (PGPR) are known to influence plant growth by various director indirect mechanisms. Thirty–two strains were isolated from 15 soils sampled in central Iran, byusing and comparing three different methods. The screening of soil samples by means of soil paste–plate method combined with isolation on mannitol agar proved to be the best strategy in terms ofreliabil...
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ژورنال
عنوان ژورنال: Cytometry Part A
سال: 2018
ISSN: 1552-4922,1552-4930
DOI: 10.1002/cyto.a.23631